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Gamma Tubulin Mouse mAb[70F5]Cat NO.: A70220

Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using Gamma Tubulin Mouse mAb IgG [70F5] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TUBG1,TUBG,TBG1_HUMAN,Tubulin gamma-1 chain

UniProtID :P23258

MASS(da) :51,170

MW(kDa) :55kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Gamma Tubulin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NCCIT cells

Function : Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.