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GCLM Mouse mAb[9L3T]Cat NO.: A22210

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GCLM Mouse mAb IgG [9L3T] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GCLM,GLCLR,GSH0_HUMAN,Glutamate--cysteine ligase regulatory subunit

UniProtID :P48507

MASS(da) :30,727

MW(kDa) :31kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GCLM.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Tissue specificity :In all tissues examined. Highest levels in skeletal muscle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.