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Western blot(SDS PAGE) analysis of extracts from rat brain tissue.Using GFAP Mouse mAb IgG [D9Q4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GFAP,GFAP_HUMAN,Glial fibrillary acidic protein
UniProtID :P14136
MASS(da) :49,880
MW(kDa) :52kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GFAP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :rat brain tissue
Function : GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificity :Expressed in cells lacking fibronectin..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.