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GNL3 Mouse mAb[860E]Cat NO.: A98580

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GNL3 Mouse mAb IgG [860E] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GNL3,E2IG3,NS,GNL3_HUMAN,Guanine nucleotide-binding protein-like 3

UniProtID :Q9BVP2

MASS(da) :61,993

MW(kDa) :62kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GNL3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity)..

Tissue specificity :Increased levels in lung tissue in cancer patients..

Subcellular locationi :Nucleus. Nucleus, nucleolus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.