GOLPH3 Mouse mAb[1502]Cat NO.: A56718

Protein names ：GOLPH3,GPP34,GOLP3_HUMAN,Golgi phosphoprotein 3

UniProtID ：Q9H4A6

MASS(da) ：33,811

MW(kDa) ：34kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human GOLPH3.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis..

Tissue specificity ：Detected in muscle fibers of patients with mitochondrial diseases,not detected in normal muscle fibers.

Subcellular locationi ：Golgi apparatus, Golgi stack membrane,Peripheral membrane protein,Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane,Peripheral membrane protein,Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.