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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HNRNPH1 Mouse mAb IgG [V2HS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HNRNPH1,HNRPH,HNRPH1,HNRH1_HUMAN,Heterogeneous nuclear ribonucleoprotein H [Cleaved into: Heterogeneous nuclear ribonucleoprotein H, N-terminally processed]
UniProtID :P31943
MASS(da) :49,229
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HNRNPH1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG)..
Tissue specificity :Expressed ubiquitously.
Subcellular locationi :Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.