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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using HVCN1 Mouse mAb IgG [HNV7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HVCN1,VSOP,UNQ578/PRO1140,HVCN1_HUMAN,Voltage-gated hydrogen channel 1
UniProtID :Q96D96
MASS(da) :31,683
MW(kDa) :28 35kDa,40kDa,60kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HVCN1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis..
Tissue specificity :Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen..
Subcellular locationi :Membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.