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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using KIF20A Mouse mAb IgG [M891] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KIF20A,MKLP2,RAB6KIFL,KI20A_HUMAN,Kinesin-like protein KIF20A
UniProtID :O95235
MASS(da) :100,278
MW(kDa) :100kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human KIF20A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility..
Subcellular locationi :Golgi apparatus. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.