Kininogen 1 Mouse mAb[6198]Cat NO.: A46489

Protein names ：KNG1,BDK,KNG,KNG1_HUMAN,Kininogen-1 [Cleaved into: Kininogen-1 heavy chain,T-kinin ,Bradykinin ,Lysyl-bradykinin ,Kininogen-1 light chain,Low molecular weight growth-promoting factor]

UniProtID ：P01042

MASS(da) ：71,957

MW(kDa) ：68kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human Kininogen 1.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：human heart tissue

Function ： (1) Kininogens are inhibitors of thiol proteases,(2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII,(3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes,(4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action),(5) LMW-kininogen inhibits the aggregation of thrombocytes,(6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

Tissue specificity ：Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors..

Subcellular locationi ：Secreted, extracellular space.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.