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MDH1 Mouse mAb[3AKJ]Cat NO.: A85426

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MDH1 Mouse mAb IgG [3AKJ] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 100ul2088.00库存充足
  • 200ul3098.00库存充足
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Product information

Protein names :MDH1,MDHA,MDHC_HUMAN,Malate dehydrogenase, cytoplasmic

UniProtID :P40925

MASS(da) :36,426

MW(kDa) :36kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MDH1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237)..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.