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Western blot(SDS PAGE) analysis of extracts from L02 cells.Using MFF Mouse mAb IgG [MRDB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MFF,C2orf33,AD030,AD033,GL004,MFF_HUMAN,Mitochondrial fission factor
UniProtID :Q9GZY8
MASS(da) :38,465
MW(kDa) :26-29kDa,35-38kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MFF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :L02 cells
Function : Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles..
Tissue specificity :Highly expressed in heart, kidney, liver, brain, muscle, and stomach..
Subcellular locationi :Mitochondrion outer membrane,Single-pass type IV membrane protein. Peroxisome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.