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Western blot(SDS PAGE) analysis of extracts from PC-12 cells.Using MMP3 Mouse mAb IgG [N342] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MMP3,STMY1,MMP3_HUMAN,Stromelysin-1
UniProtID :P08254
MASS(da) :53,977
MW(kDa) :60kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MMP3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-12 cells
Function : Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V,collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.