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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MRPS25 Mouse mAb IgG [N8X2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MRPS25,RPMS25,RT25_HUMAN,28S ribosomal protein S25, mitochondrial
UniProtID :P82663
MASS(da) :20,116
MW(kDa) :20kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MRPS25.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.