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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using MTHFR Mouse mAb IgG [3XD0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MTHFR,MTHR_HUMAN,Methylenetetrahydrofolate reductase
UniProtID :P42898
MASS(da) :74,597
MW(kDa) :70kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MTHFR.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine (PubMed:29891918). Represents a key regulatory connection between the folate and methionine cycles (Probable)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.