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Western blot(SDS PAGE) analysis of extracts from COLO 320 cells.Using MYH14 Mouse mAb IgG [CMP0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYH14,KIAA2034,FP17425,MYH14_HUMAN,Myosin-14
UniProtID :Q7Z406
MASS(da) :227,871
MW(kDa) :230kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MYH14.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :COLO 320 cells
Function : Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping..
Tissue specificity :High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.