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Western blot(SDS PAGE) analysis of extracts from U2OS cells.Using NAGA Mouse mAb IgG [7TZ6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NAGA,NAGAB_HUMAN,Alpha-N-acetylgalactosaminidase
UniProtID :P17050
MASS(da) :46,565
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NAGA.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U2OS cells
Function : Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids..
Subcellular locationi :Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.