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Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using NBR1 Mouse mAb IgG [355U] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NBR1,1A13B,KIAA0049,M17S2,MIG19,NBR1_HUMAN,Next to BRCA1 gene 1 protein
UniProtID :Q14596
MASS(da) :107,413
MW(kDa) :140kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NBR1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NCCIT cells
Function : Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets..
Subcellular locationi :Cytoplasm. Cytoplasmic vesicle, autophagosome. Lysosome. Cytoplasm, myofibril, sarcomere, M line.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.