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NDUFS3 Mouse mAb[39YX]Cat NO.: A12299

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using NDUFS3 Mouse mAb IgG [39YX] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NDUFS3,NDUS3_HUMAN,NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

UniProtID :O75489

MASS(da) :30,242

MW(kDa) :30kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NDUFS3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060). Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060)..

Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.