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NF-L Mouse mAb[K9PK]Cat NO.: A41246

Western blot(SDS PAGE) analysis of extracts from PC-12 cells.Using NF-L Mouse mAb IgG [K9PK] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NEFL,NF68,NFL,NFL_HUMAN,Neurofilament light polypeptide

UniProtID :P07196

MASS(da) :61,517

MW(kDa) :65kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NF-L.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :PC-12 cells

Function : Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)..

Subcellular locationi :Cell projection, axon. Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.