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Western blot(SDS PAGE) analysis of extracts from HSC-T6 cells.Using NFS1 Mouse mAb IgG [Z0RH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NFS1,NIFS,HUSSY-08,NFS1_HUMAN,Cysteine desulfurase, mitochondrial
UniProtID :Q9Y697
MASS(da) :50,196
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NFS1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HSC-T6 cells
Function : Catalyzes the removal of elemental sulfur from cysteine to produce alanine (PubMed:18650437). It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (PubMed:18650437)..
Tissue specificity :Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas..
Subcellular locationi :[Isoform Mitochondrial]: Mitochondrion.,[Isoform Cytoplasmic]: Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.