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NHE1 Mouse mAb[DVGS]Cat NO.: A16992

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using NHE1 Mouse mAb IgG [DVGS] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SLC9A1,APNH1,NHE1,SL9A1_HUMAN,Sodium/hydrogen exchanger 1 /H antiporter, amiloride-sensitive) /H exchanger 1)

UniProtID :P19634

MASS(da) :90,763

MW(kDa) :110kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NHE1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction..

Tissue specificity :Kidney and intestine.

Subcellular locationi :Membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.