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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using NMT1 Mouse mAb IgG [E5WI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NMT1,NMT,NMT1_HUMAN,Glycylpeptide N-tetradecanoyltransferase 1
UniProtID :P30419
MASS(da) :56,806
MW(kDa) :60kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NMT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed:22865860, PubMed:25255805, PubMed:9353336, PubMed:9506952). Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed:32103017, PubMed:32111831). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed:32103017)..
Tissue specificity :Heart, gut, kidney, liver and placenta..
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.