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Western blot(SDS PAGE) analysis of extracts from HSCT6 cells.Using P4HA2 Mouse mAb IgG [ZNR9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :P4HA2,UNQ290/PRO330,P4HA2_HUMAN,Prolyl 4-hydroxylase subunit alpha-2
UniProtID :O15460
MASS(da) :60,902
MW(kDa) :61kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human P4HA2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HSCT6 cells
Function : Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins..
Tissue specificity :Expressed in the heart, placenta, lung and pancreas..
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.