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PEDF Mouse mAb[WB2E]Cat NO.: A14282

Western blot(SDS PAGE) analysis of extracts from human plasma tissue.Using PEDF Mouse mAb IgG [WB2E] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SERPINF1,PEDF,PIG35,PEDF_HUMAN,Pigment epithelium-derived factor

UniProtID :P36955

MASS(da) :46,312

MW(kDa) :46kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PEDF.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human plasma tissue

Function : Neurotrophic protein,induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity..

Tissue specificity :Retinal pigment epithelial cells and blood plasma..

Subcellular locationi :Secreted. Melanosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.