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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PGAM1 Mouse mAb IgG [OCE1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PGAM1,PGAMA,CDABP0006,PGAM1_HUMAN,Phosphoglycerate mutase 1
UniProtID :P18669
MASS(da) :28,804
MW(kDa) :29kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PGAM1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate (PubMed:23653202). Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate (PubMed:23653202)..
Tissue specificity :Expressed in the liver and brain. Not found in the muscle..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.