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Western blot(SDS PAGE) analysis of extracts from Caco-2 cells.Using PLEKHB2 Mouse mAb IgG [ILUP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PLEKHB2,EVT2,PKHB2_HUMAN,Pleckstrin homology domain-containing family B member 2
UniProtID :Q96CS7
MASS(da) :24,736
MW(kDa) :19-25;30-34kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PLEKHB2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Caco-2 cells
Function : Involved in retrograde transport of recycling endosomes..
Subcellular locationi :Recycling endosome membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.