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PPT1 Mouse mAb[K1DY]Cat NO.: A79158

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using PPT1 Mouse mAb IgG [K1DY] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PPT1,CLN1,PPT,PPT1_HUMAN,Palmitoyl-protein thioesterase 1

UniProtID :P50897

MASS(da) :34,193

MW(kDa) :32-38kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PPT1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons (PubMed:8816748)..

Subcellular locationi :Lysosome. Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.