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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using PPT1 Mouse mAb IgG [K1DY] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PPT1,CLN1,PPT,PPT1_HUMAN,Palmitoyl-protein thioesterase 1
UniProtID :P50897
MASS(da) :34,193
MW(kDa) :32-38kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PPT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons (PubMed:8816748)..
Subcellular locationi :Lysosome. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.