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PURA Mouse mAb[3001]Cat NO.: A29658

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using PURA Mouse mAb IgG [3001] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PURA,PUR1,PURA_HUMAN,Transcriptional activator protein Pur-alpha

UniProtID :Q00577

MASS(da) :34,911

MW(kDa) :35kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PURA.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.