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Western blot(SDS PAGE) analysis of extracts from U2OS cells.Using RAB2A Mouse mAb IgG [EDJR] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAB2A,RAB2,RAB2A_HUMAN,Ras-related protein Rab-2A
UniProtID :P61019
MASS(da) :23,546
MW(kDa) :21kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RAB2A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U2OS cells
Function : The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi..
Subcellular locationi :Endoplasmic reticulum-Golgi intermediate compartment membrane,Lipid-anchor. Melanosome. Endoplasmic reticulum membrane,Lipid-anchor. Golgi apparatus membrane,Lipid-anchor. Cytoplasmic vesicle, secretory vesicle, acrosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.