RANKL Mouse mAb[6GV8]Cat NO.: A53841

Protein names ：TNFSF11,OPGL,RANKL,TRANCE,TNF11_HUMAN,Tumor necrosis factor ligand superfamily member 11 [Cleaved into: Tumor necrosis factor ligand superfamily member 11, membrane form,Tumor necrosis factor ligand superfamily member 11, soluble form]

UniProtID ：O14788

MASS(da) ：35,478

MW(kDa) ：35kda

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human RANKL.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：COLO 320 cells

Function ： Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity)..

Tissue specificity ：Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.

Subcellular locationi ：[Isoform 1]: Cell membrane,Single-pass type II membrane protein.,[Isoform 3]: Cell membrane,Single-pass type II membrane protein.,[Isoform 2]: Cytoplasm.,[Tumor necrosis factor ligand superfamily member 11, soluble form]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.