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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using RNASEH2A Mouse mAb IgG [SU2Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RNASEH2A,RNASEHI,RNHIA,RNH2A_HUMAN,Ribonuclease H2 subunit A )
UniProtID :O75792
MASS(da) :33,395
MW(kDa) :33kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RNASEH2A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.