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Western blot(SDS PAGE) analysis of extracts from human plasma.Using SERPIND1 Mouse mAb IgG [2PD4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SERPIND1,HCF2,HEP2_HUMAN,Heparin cofactor 2
UniProtID :P05546
MASS(da) :57,071
MW(kDa) :66kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SERPIND1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human plasma
Function : Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.., Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils..
Tissue specificity :Expressed predominantly in liver. Also present in plasma.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.