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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using SHMT1 Mouse mAb IgG [91X5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SHMT1,GLYC_HUMAN,Serine hydroxymethyltransferase, cytosolic
UniProtID :P34896
MASS(da) :53,083
MW(kDa) :45kDa,50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SHMT1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Interconversion of serine and glycine (PubMed:8505317, PubMed:24698160)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.