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Western blot(SDS PAGE) analysis of extracts from rat colon tissue.Using SIAE Mouse mAb IgG [57P1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SIAE,YSG2,SIAE_HUMAN,Sialate O-acetylesterase
UniProtID :Q9HAT2
MASS(da) :58,315
MW(kDa) :70kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SIAE.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :rat colon tissue
Function : Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.
Tissue specificity :Widely expressed with high expression in the testis, prostate, and colon..
Subcellular locationi :Lysosome. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.