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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using SLC25A17 Mouse mAb IgG [FMJ9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC25A17,PMP34,PM34_HUMAN,Peroxisomal membrane protein PMP34
UniProtID :O43808
MASS(da) :34,567
MW(kDa) :35kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SLC25A17.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Peroxisomal transporter for multiple cofactors like coenzyme A (CoA), flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and nucleotide adenosine monophosphate (AMP), and to a lesser extent for nicotinamide adenine dinucleotide (NAD(+)), adenosine diphosphate (ADP) and adenosine 3',5'-diphosphate (PAP). May catalyze the transport of free CoA, FAD and NAD(+) from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. Inhibited by pyridoxal 5'-phosphate and bathophenanthroline in vitro..
Tissue specificity :Ubiquitous. Expressed in liver..
Subcellular locationi :Cytoplasm. Peroxisome membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.