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Western blot(SDS PAGE) analysis of extracts from human ileum tissue.Using SMMHC Mouse mAb IgG [O1B5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYH11,KIAA0866,MYH11_HUMAN,Myosin-11
UniProtID :P35749
MASS(da) :227,339
MW(kDa) :220kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SMMHC.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human ileum tissue
Function : Muscle contraction.
Tissue specificity :Smooth muscle,expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles..
Subcellular locationi :Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.