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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using SPTBN1 Mouse mAb IgG [MWXE] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SPTBN1,SPTB2,SPTB2_HUMAN,Spectrin beta chain, non-erythrocytic 1
UniProtID :Q01082
MASS(da) :274,609
MW(kDa) :275kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SPTBN1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Tissue specificity :Isoform 2 is present in brain, lung and kidney (at protein level)..
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, M line.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.