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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using SQLE Mouse mAb IgG [WOR3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SQLE,ERG1,ERG1_HUMAN,Squalene monooxygenase
UniProtID :Q14534
MASS(da) :63,923
MW(kDa) :64kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human SQLE.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis..
Tissue specificity :Detected in liver (at protein level)..
Subcellular locationi :Microsome membrane,Peripheral membrane protein. Endoplasmic reticulum membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.