TH Mouse mAb[08E9]Cat NO.: A98142

Protein names ：TH,TYH,TY3H_HUMAN,Tyrosine 3-monooxygenase

UniProtID ：P07101

MASS(da) ：58,600

MW(kDa) ：55kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human TH.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：PC-12 cells

Function ： Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:17391063, PubMed:1680128, PubMed:15287903, PubMed:8528210, Ref.18, PubMed:34922205, PubMed:24753243). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).., [Isoform 5]: Lacks catalytic activity.., [Isoform 6]: Lacks catalytic activity..

Tissue specificity ：Mainly expressed in the brain and adrenal glands.

Subcellular locationi ：Cytoplasm, perinuclear region. Nucleus. Cell projection, axon. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.