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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using Tim23 Mouse mAb IgG [MIH7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TIMM23,TIM23,TIM23_HUMAN,Mitochondrial import inner membrane translocase subunit Tim23
UniProtID :O14925
MASS(da) :21,943
MW(kDa) :21kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Tim23.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane..
Subcellular locationi :Mitochondrion inner membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.