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TMEM74 Mouse mAb[3493]Cat NO.: A28431

Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using TMEM74 Mouse mAb IgG [3493] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TMEM74,TMM74_HUMAN,Transmembrane protein 74

UniProtID :Q96NL1

MASS(da) :33,338

MW(kDa) :33kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TMEM74.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :LNCaP cells

Function : Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction..

Tissue specificity :Expressed in heart, lung, and placenta..

Subcellular locationi :Lysosome membrane,Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.