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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using TMEM74 Mouse mAb IgG [3493] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TMEM74,TMM74_HUMAN,Transmembrane protein 74
UniProtID :Q96NL1
MASS(da) :33,338
MW(kDa) :33kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TMEM74.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction..
Tissue specificity :Expressed in heart, lung, and placenta..
Subcellular locationi :Lysosome membrane,Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.