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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using TMF1 Mouse mAb IgG [250V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TMF1,ARA160,TMF1_HUMAN,TATA element modulatory factor
UniProtID :P82094
MASS(da) :122,842
MW(kDa) :150kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TMF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP)..
Subcellular locationi :Cytoplasm. Nucleus. Golgi apparatus membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.