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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using TOE1 Mouse mAb IgG [E2VZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TOE1,TOE1_HUMAN,Target of EGR1 protein 1
UniProtID :Q96GM8
MASS(da) :56,548
MW(kDa) :57kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TOE1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing (PubMed:28092684)..
Tissue specificity :Widely expressed..
Subcellular locationi :Nucleus, nucleolus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.