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Tom22 Mouse mAb[KHHZ]Cat NO.: A14906

Western blot(SDS PAGE) analysis of extracts from A431 cells.Using Tom22 Mouse mAb IgG [KHHZ] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TOMM22,TOM22,TOM22_HUMAN,Mitochondrial import receptor subunit TOM22 homolog

UniProtID :Q9NS69

MASS(da) :15,522

MW(kDa) :22kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Tom22.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cells

Function : Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed:10982837). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity)..

Tissue specificity :Ubiquitous.

Subcellular locationi :Mitochondrion outer membrane,Single-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.