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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using VAMP3/Cellubrevin Mouse mAb IgG [99SZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VAMP3,SYB3,VAMP3_HUMAN,Vesicle-associated membrane protein 3
UniProtID :Q15836
MASS(da) :11,309
MW(kDa) :17kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human VAMP3/Cellubrevin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network..
Subcellular locationi :Membrane,Single-pass type IV membrane protein. Cell junction, synapse, synaptosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.