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VAPB Mouse mAb[1JXH]Cat NO.: A11287

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using VAPB Mouse mAb IgG [1JXH] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :VAPB,UNQ484/PRO983,VAPB_HUMAN,Vesicle-associated membrane protein-associated protein B/C

UniProtID :O95292

MASS(da) :27,228

MW(kDa) :30kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human VAPB.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation..

Tissue specificity :Ubiquitous. Isoform 1 predominates.

Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type IV membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.