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Western blot(SDS PAGE) analysis of extracts from U-251 cells.Using WASF3 Mouse mAb IgG [4N0B] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :WASF3,KIAA0900,SCAR3,WAVE3,WASF3_HUMAN,Wiskott-Aldrich syndrome protein family member 3
UniProtID :Q9UPY6
MASS(da) :55,293
MW(kDa) :50-55and66-70kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human WASF3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-251 cells
Function : Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape..
Tissue specificity :Expressed in ovary and brain.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.