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ABHD5 Mouse mAb[2R57]Cat NO.: A37239

Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using ABHD5 Mouse mAb IgG [2R57] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ABHD5,NCIE2,CGI-58,ABHD5_HUMAN,1-acylglycerol-3-phosphate O-acyltransferase ABHD5

UniProtID :Q8WTS1

MASS(da) :39,096

MW(kDa) :39kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ABHD5.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :LNCaP cells

Function : Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid (PubMed:18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion (By similarity)..

Tissue specificity :Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level)..

Subcellular locationi :Cytoplasm. Lipid droplet. Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.