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Amphiregulin Mouse mAb[55K9]Cat NO.: A94781

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using Amphiregulin Mouse mAb IgG [55K9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :AREG,AREGB,SDGF,AREG_HUMAN,Amphiregulin

UniProtID :P15514

MASS(da) :27,895

MW(kDa) :50kDa,37kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Amphiregulin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Subcellular locationi :Membrane,Single-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.