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eIF4G Rabbit mAb [V39R]Cat NO.: A98262

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using eIF4G Rabbit mAb [V39R] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EIF4G1,EIF4F,EIF4G,EIF4GI,IF4G1_HUMAN,Eukaryotic translation initiation factor 4 gamma 1

UniProtID :Q04637

MASS(da) :175,491

MW(kDa) :220 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human eIF4G

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139)..

Subcellular locationi :Cytoplasm, Stress granule.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.